Extended Data Fig. 7: Mouse 3D in vitro experimental analysis.
From: Targeting circulating mechanoresponsive monocytes and macrophages to reduce fibrosis
(a) WT murine dermal fibroblasts and GFP+ murine myeloid cells harvested. (b) Violin plots of common fibroblast markers in our co-cultured fibroblasts. (c) FACS gates of co-culture collagen scaffolds subjected to no strain, strain, and strain + FAKI treatment. (d) Confocal imaging and quantification showing the expression of thrombospondin (red) and DAPI (blue) (*p = 0.0389 NS vs S; *p = 0.0402 NS vs S + F; **p = 0.0013 S vs S + F). Scale bar = 200um. Statistical comparisons made using one-way analysis of variance (ANOVA) with Tukey’s multiple comparisons tests. All data represent mean ± SEM of biological replicates. Panel a created with BioRender.com.
