Extended Data Fig. 4: ROSA26GFPm-OVA mice.
From: Phagocytic clearance of targeted cells with a synthetic ligand
a, Schematic of the ROSA26GFPm-OVA mouse model. The CMV enhancer, chicken β-actin promoter, GFPm-OVA fusion protein (linked by GGGGS) and rabbit β-globin polyadenylation signal (pA) were inserted into the Rosa26 locus of C57BL/6 mice. b, GFP expression in ROSA26GFPm-OVA mice. GFP expression was analyzed on thymocytes, splenocytes, bone marrow (BM), and blood cells from WT or ROSA26GFPm-OVA mice using an anti-GFP antibody by flow cytometry. c, Crunch binding to cells from ROSA26GFPm-OVA mice. Thymocytes, splenocytes, bone marrow, and blood cells from WT or ROSA26GFPm-OVA mice were incubated with or without 10 µg/ml GFPNb-Crunch, and binding was detected using an anti-FLAG antibody by flow cytometry.
