Extended Data Fig. 3: Cell survival assay.
From: Phagocytic clearance of targeted cells with a synthetic ligand
a, Experimental design. Ba/F3 cells were engineered to express MerTK, aXkr4 (to expose PtdSer), or both. 2.5 × 105 cells were cultured in 500 µl IL-3(–) RPMI1640 with FBS. MerTK activation supports survival and proliferation. b–e, Cell survival by PtdSer and MerTK. Cell number and viability were measured by Trypan blue exclusion every 24 hr. Line graphs show mean cell number (b) and viability (c); bar plots show both parameters at 48 and 72 hr (d, e) (n = 3, independent biological samples). f–i, Cell survival by Crunch. MerTK⁺ Ba/F3 cells were cultured with (+) or without (-) 10 µg/ml GFPNb-Crunch in IL-3(–) medium. Cell number (f) and viability (g) were measured every 24 hr. Bar plots show both at 48 and 72 hr (h, i) (n = 3, independent biological samples). Data are mean ± S.D. Statistical analysis: one-way ANOVA with Tukey-Kramer t-test (d–e, g); Student’s t test (f–i). N.S., P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001.
