Extended Data Fig. 5: CAR T cell phenotypes and metabolic profile before in vivo treatment course. | Nature Biomedical Engineering

Extended Data Fig. 5: CAR T cell phenotypes and metabolic profile before in vivo treatment course.

From: Label-free metabolic imaging monitors the fitness of chimeric antigen receptor T cells

Extended Data Fig. 5

(a) Experimental timeline. (b) Phenotypes of CAR+ T cells expanded in Imm, Tex→Imm, and Tex conditions based on expression of 7 surface markers. (c) Extracellular flux analysis of baseline oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) from CD3 T cells that were unactivated (quiescent) or activated and expanded in Imm or Tex→Imm condition. n = 9–18 samples/condition. Brown-Forsythe and Welch ANOVA tests with Dunnett’s T3 post hoc test for multiple comparisons. Bars are mean ± SD. * p < 0.0001.

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