Extended Data Fig. 6: Expansion conditions shaped T cell expansion capacity and metabolism.
From: Label-free metabolic imaging monitors the fitness of chimeric antigen receptor T cells
(a, b) Imm and Tex-Imm T cells exhibited high expansion capacity. Fold expansion of T cells from two healthy donors under various expansion conditions (a) throughout and (b) on day 8 of the expansion process n = 4-6 replicates/condition across two donors, two way ANOVA test with Tukey’s posthoc test for multiple comparisons. (c) ATP production by T cells under several expansion conditions. (d–m) Media transition from TexMACs to ImmunoCult XF (Tex-Imm) shifted T cell metabolism towards balanced oxidative phosphorylation while maintaining ATP production. (d) Representative NAD(P)H τm images of T cells on day 9. (e) Quantification of NAD(P)H τm and (f) normalized NAD(P)H intensity of expanded T cells from two healthy donors on day 9. n = 361-580 cells/conditions across 2 donors, color coded by donors. (g, h) Metabolic cofactors, (i–m) dehydrogenases activity measured from expanded T cells of two healthy donors on day 9. n = 6 replicates across 2 donors. Brown-Forsythe and Welch ANOVA test with Dunnett’s T3 post hoc test for multiple comparisons. Bars are mean ± SD. * p < 0.0001.
