Extended Data Fig. 7: In vivo treatment response and phenotyping analysis of CAR T cells post in vivo treatment.
From: Label-free metabolic imaging monitors the fitness of chimeric antigen receptor T cells
(a) Experimental timeline. (b) Fold change in tumor flux (measured with IVIS imaging) in NSG tumor-bearing mice after CAR T cell treatment. Dash line represents no change in tumor flux (fold change = 1). (c) CAR T treatment outcomes on day 17 post treatment. (d–g) Expression of stem central memory (d, e) and exhaustion phenotypes (f, g) in CAR T cells isolated from treated NSG mouse spleen at day 21 post-treatment. (d, f) Representative flow histograms from one individual mouse in each treatment group. (e, g) Quantification of stem central memory (CD62 + CCR7+) and exhausted (PD-1 + TIGIT+) populations in treated mouse spleen. n = 12 mice, color coded by CAR T treatment groups. Two-sided non-parametric Mann-Whitney test. Bars are mean ± SD.
