Extended Data Fig. 1: Detection of prime editing by ISS in cultured cells.
From: Spatial profiling of gene editing by in situ sequencing in mice and macaques
(a) Schematic of the experimental setup to detect prime editing in GFP reporter cells treated with PE2. (b) Quantification of the percentage of edited reads using NGS. Each datapoint represents an independent well of separately transfected cells. (c) Image shows edited (green) and unedited GFP reporter cells (left panel), and RCPs (right panel). Scale bar: 10 um. (d) Quantification of the fraction of GFP positive cells and edited reads detected using ISS. Each point represents an independent well (replicate 1, n = 805 cells; replicate 2 = 704 cells). (e) Quantification of the concordance between GFP expression and ISS in prime edited GFP reporter cells.
