Extended Data Fig. 2: Detection of base editing by ISS in the mouse brain after intracranial AAV injection.
From: Spatial profiling of gene editing by in situ sequencing in mice and macaques
(a) (Left) Schematic of the experimental set-up. 2 μl AAV PHP.eB with a concentration of 1×1013 vg/mL encoding for the ActbV30 targeting ABEmax were injected into the hypothalamus of adult mice (Right). Image from the Allen Brain Atlas. Depicting areas illustrate the areas analyzed by NGS (yellow) or ISS (red). (b) Representative brain tissue region cut by laser microdissection from mice injected in hypothalamus. (c) Quantification of editing efficiencies in the hypothalamus of treated mice detected by NGS and ISS. Datapoints represent different mice. n = 3922 and 1703 RCPs. (d) Image shows RCPs in the hypothalamus of mice treated with ABE targeting ActbV30. Insets show the RCPs as single channel images. Scale bar: 10 um. (e) Quantification of editing rates in distinct sub-regions of the hypothalamus. Datapoints represent separate areas in the hypothalamus from at least two mice. Left: n = 521 and 659 RCPs; mid: n = 1623 and 807 RCPs; right: n = 1778 and 237 RCPs. Bars represent mean ± s.d. a, Created with BioRender.com.
