Extended Data Fig. 3: Biodistribution of AAV9 and RNA-LNP in mice liver.
From: Spatial profiling of gene editing by in situ sequencing in mice and macaques
(a) (Top) Schematic of the experimental set-up. Adult mice were injected via the tail-vein with either AAV9 TagRFP-expressing vector or LNP-mCherry. (Bottom) Representative images of the liver lobule zonation from treated mice. Pericentral cells are counterstained with Glutamine Synthetase (GS) (magenta) antibody and periportal cells are counterstained with ECAD (green) antibody. Scale bar: 100 um. (b) Quantification of average intensities in arbitrary units of either TagRFP or mCherry in the three liver zones. Datapoints represent independently treated animals. a, Created with BioRender.com.
