Extended Data Fig. 5: Endothelial cell derivation. | Nature Biomedical Engineering

Extended Data Fig. 5: Endothelial cell derivation.

From: Engineered human induced pluripotent stem cell models reveal altered podocytogenesis in congenital heart disease-associated SMAD2 mutations

Extended Data Fig. 5: Endothelial cell derivation.

a, RNA expression of mesenchymal markers ICAM1, Smoothelin, and Vimentin in WT and mutant endothelial cells. Data are mean values ± SEM; n = 3 independent experiments. ICAM1 was increased in SMAD2−/− (P = 0.0009) and SMAD2mh1/mh1 (P = 0.0078) endothelial cells. Smoothelin was increased in SMAD2−/− (P < 0.0001) and SMAD2mh1/mh1 (P = 0.0020) endothelial cells. Vimentin was increased in SMAD2−/− (P < 0.0001) and SMAD2mh1/mh1 (P < 0.0001) endothelial cells. We performed a P < 0.05 one-way ANOVA with Dunnett’s multiple comparison test. Same colors indicate 3-4 independent wells of endothelial cells from each biological replicate. b, Representative immunofluorescent images showing the expression of mesenchymal markers Transgelin (cyan) and TWIST (red) in endothelial cells after 2 weeks of maintenance culture. n = 2 independent experiments. Scale bar, 100 µm.

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