Extended Data Fig. 4: Interface integration with hCO.

a, Bright field images of generated hCOs. Images are representative of 6 organoids. b, Immunostaining images of day 30 hCO. c, Immunostaining images of day 120 hCO. Immunostaining images are representative images of 3 organoids. d, Bright field images monitoring the grow-in-place process of one integrated hCO. This experiment was repeated with 6 devices. e, Electrophysiological recording of the same hCO in (d) showing increasing number of active channels following the filling of gaps along the interface from day 64 to day 79. f, Immunostaining images of control group hCO and hCO grown-in-place for two weeks show negligible interface effect on cell distributions. Images are representative of 3 sets of organoids.