Extended Data Fig. 7: Generation and characterization of Otof-1233delC mouse line.
From: Template-independent genome editing and restoration for correcting frameshift disorders
a, Four-color chromatogram showing the genotypes of WT, Otof1233delC/+, and Otof1233delC/1233delC mice. b, Western blot analysis of Otoferlin protein in cochleae using an Otoferlin antibody from control and Otof1233delC/1233delC mice, with GAPDH serving as an internal control. c, Whole mount immunostaining of WT (c) and of Otof1233delC/1233delC (d) cochleae. Otoferlin (red), Myo7a (green), and DAPI (blue) signals are shown. Lower panels highlight magnified regions (white frame) from the upper panels. White frames in lower panels highlight Otoferlin-positive IHCs in WT cochleae (c) and Otoferlin-negative IHCs in Otof1233delC/1233delC cochleae (d). Scale bars: 100 μm (upper panels), 20 μm (lower panels). e, Click ABR waveforms from WT (left) and Otof1233delC/1233delC (right) mice. f, Quantification of click and pure-tone ABR thresholds for WT (gray) and Otof1233delC/1233delC (purple) mice.
