Supplementary Figure 2: Identification of exomeres and exosome subpopulations released by multiple cancer cell lines. | Nature Cell Biology

Supplementary Figure 2: Identification of exomeres and exosome subpopulations released by multiple cancer cell lines.

From: Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation

Supplementary Figure 2

Shown are AF4 profiles (a) and representative TEM images (b) of unfractionated input samples and pooled fractions of exomeres, Exo- S and Exo-L that derived from various cancer cell lines, including AsPC-1, Pan02, MDA-MB-231-4175, and 4T1. Multiple independent experiments were conducted with similar results for (a) (repeated times: AsPC-1, 9x; Pan02, 16x; 4175, 17x; 4T1, 10x) and (b) (AsPC-1, 3x; Pan02, 2x; 4175, 1x; 4T1, 4x). Scale bar, 200 nm. x- axis, time (min); y-axis (scale) and black dots, hydrodynamic radius (nm); Red and blue lines illustrate the QELS (DLS) intensity and UV absorbance (shown on a relative scale), respectively.

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