Supplementary Figure 6: Expression of PSPC1 is required for TGFβ1–enhanced EMT and stemness. | Nature Cell Biology

Supplementary Figure 6: Expression of PSPC1 is required for TGFβ1–enhanced EMT and stemness.

From: PSPC1 mediates TGF-β1 autocrine signalling and Smad2/3 target switching to promote EMT, stemness and metastasis

Supplementary Figure 6: Expression of PSPC1 is required for TGFβ1–enhanced EMT and stemness.The alternative text for this image may have been generated using AI.

. (a-d) PSPC1 increased cell migration (a), spheroid formation (b), and expression (c) and (d) binding to the promoters of EMT-TFs and CSC-TFs could be suppressed by treatment of 20 mM SB431542 (data representative of 3 independent experiments). (e-j) TGFβ1 (5 ng/ml, 24 hr) treatments induced cell migration (e, h), spheroids formation (f, i) and upregulation of EMT-TFs and CSCs-TFs expression (g, j) in A549 cells (e-g) and MDAMB231 (h-j) were abolished by knocking down PSPC1. All error bars are mean +/- sd, n = 3 independent experiments. *P<0.05, **P<0.001, and ***P<0.0001, determined by two-tailed Student’s t-test (95% confidence interval). The source data are provided in Supplementary Table 3.

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