Supplementary Figure 5: Fractal analysis of ENFs organizations during development. and subsampled fractal analysis. | Nature Cell Biology

Supplementary Figure 5: Fractal analysis of ENFs organizations during development. and subsampled fractal analysis.

From: Two succeeding fibroblastic lineages drive dermal development and the transition from regeneration to scarring

Supplementary Figure 5

a, Graphical representation of the quantitative assessment of complexity and porosity in 2D images by Fractal analysis. b, Representative confocal images of clumped posterior E11.5 EPFs (up), and stretched anterior E12.5 (down) from En1Cre;R26VT2/GK3 embryos. c, Fractal dimension (up) and lacunarity (down) values derived from ENFs of different regions and developmental stages. Mean ± SEM. n?=?9 optical fields of anterior or posterior regions at each developmental stage. RM ANOVA, p?<?0.05, Newman-Keuls test. d, Subsampled (local) fractal analysis (right) of low-power magnification confocal images of E16.5 En1Cre;R26VT2/GK3 embryos (left). Scale: 50?µm. e, Fractal dimension (up) and lacunarity (down) values derived from ENFs of different regions and developmental stages, n?>?60 subsampled values pooled from three confocal images. ANOVA, Tukey test, *p?<?0.05. Box and whiskers plots with minimum, lower quartile, median, upper quartile, and maximum. The exact p values are listed in Supplementary Table 1.

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