Supplementary Figure 6: Fibronectin matrix stiffens concurrent with EPF expansion.

a–d, Immunofluorescence for Fibronectin in E11.5 (a), E12.5 (b), E14.5 (c), or E16.5 (d) En1Cre;R26VT2/GK3 embryos. e–h, Representative linear profiles of diagonal lines on the Fibronectin signal from E11.5 (e), E12.5 (f), E14.5 (g), or E16.5 (h) images. Dashed lines above the plots represent the calculated distances between fibers. Scale: 50?µm. Images are representatives of 3 optical fields.