Supplementary Figure 2: Expression of mesoderm markers on ENFs and EPFs by immunofluorescence staining. | Nature Cell Biology

Supplementary Figure 2: Expression of mesoderm markers on ENFs and EPFs by immunofluorescence staining.

From: Two succeeding fibroblastic lineages drive dermal development and the transition from regeneration to scarring

Supplementary Figure 2

Cryosections were prepared from En1Cre;R26VT2/GK3 neonates (P1), and stained with primary antibodies against mouse FABP4 (a), Dlk1 (b), Sca1 (c), aSMA (d), TNC (e), and CD26 (f), and AF647-conjugated respective secondary antibodies. Depicted are representative high-power images of AF647 channel for antibodies (left), GFP-RFP channels for ENFs-EPFs (middle), and merged channels (right). Dotted lines indicate the border of epidermis and dermis, and the border of upper dermis (UD) and lower dermis (LD). Squares indicate the locations of enlarged inserts in merged images. Scales: 50?µm. The depicted are representative images of three individual embryos.

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