Supplementary Figure 2: The level of different type of RNA modifications in sperm RNA fractions extracted from F0 Dnmt2+/+ and Dnmt2–/– males under ND and HFD.
The relative level of unmodified (a) C, (b) G, (c) A, (d) U in different sperm RNA fractions (15–25 nt, 30–40 nt, 40–100 nt and >100 nt and the relative level of modified nucleotide in (e) Cm, (f) Gm, (g) m1G, (h) m7G, (i) Am, (j) I, (k) Im, (l) m6A, (m) Um, (n) m5U from different sperm RNA fractions (15–25 nt, 30–40 nt, 40–100 nt and >100 nt) of F0 Dnmt2 +/+ and Dnmt2–/– males under ND and HFD. Value for each dot are generated by pooled sperm RNAs from 8 mice, as to reach enough RNA amount in each fraction to perform LC-MS/MS. Number of dots on the figure represents number of biologically independent experiments, which is also detailed in Supplementary Table 1. All data are plotted as mean ± SEM (n = number of biologically independent experiments). All statistical analysis was performed by two-tailed, one-way Anova, uncorrected Fisher's LSD. All statistic source data and P values are provided in Supplementary Table 1.
