Supplementary Figure 3: Small RNA-seq analysis of sperm RNAs from F0 Dnmt2+/+ and Dnmt2–/– males under ND and HFD. | Nature Cell Biology

Supplementary Figure 3: Small RNA-seq analysis of sperm RNAs from F0 Dnmt2+/+ and Dnmt2–/– males under ND and HFD.

From: Dnmt2 mediates intergenerational transmission of paternally acquired metabolic disorders through sperm small non-coding RNAs

Supplementary Figure 3: Small RNA-seq analysis of sperm RNAs from F0 Dnmt2+/+ and Dnmt2–/– males under ND and HFD.

The small RNA-seq quality control, statistics, and RNA profiling. (Data and images shown in this figure are representatives of two biological replicates, raw data for both replicates are archived in the Gene Expression Omnibus under accession number GSM2574268). (a-d) Electrophoretic size distribution of RNAs in (a) Dnmt2+/+ ND, (b) Dnmt2+/+ HFD, (c) Dnmt2–/– HFD, (d) Dnmt2–/– ND, analyzed by Agilent Bioanalyzer. (e) Summary of small RNA reads. Information for RNA reads annotation was analyzed by genome match information, miRNAs, piRNAs, tRNAs and rRNAs annotation according to mouse genome database (GRCm38/mm10), miRBase211, piRNA database2,3, GtRNAdb4, NCBI Nucleotide database, Ensembl mouse ncRNA database (GRCm38/mm10) and Rfam 11.0 database5. (f-i) Length distribution and pattern changes of different sncRNAs (miRNAs, piRNAs, tsRNAs and rsRNAs) in (f) Dnmt2+/+ ND, (g) Dnmt2+/+ HFD, (h) Dnmt2–/– HFD and (i) Dnmt2–/– ND sperm. (j-m) loci mapping information of rsRNAs in different rRNAs (5sRNA, 5.8sRNA, 18sRNA and 28sRNA) for (j) Dnmt2+/+ ND, (k) Dnmt2+/+ HFD, (l) Dnmt2–/– HFD and (m) Dnmt2–/– ND sperm. RPM: reads per million.

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