Supplementary Fig. 8: OCT4 Intron Elements 1 and 2.
From: TFAP2C regulates transcription in human naive pluripotency by opening enhancers
a, Regulatory elements in the proximity of OCT4 locus. The conservation track shows one dot for each base, with the degree of conservation in placental mammals indicated by the value in the Y-axis. Note higher conservation over regulatory elements and coding sequence. b, Sashimi plot showing splice events over POU5F1 locus in naive hESCs. Some RNA-seq reads are observed over the POU5F1 Intron Element 1, possibly enhancer RNA or intronic RNA, but there are no splice events linking these to OCT4 transcripts. This indicates that the Intron Element 1 is not an alternative TSS. c, Normal karyotype in intron enhancer deletant and WT control cells. d, Western blot for OCT4 in Control and ΔIntron enhancer 1 in primed conditions. Uncropped Western blot images are available in Supplementary Fig. 9.
