Supplementary Figure 5: Quantitative immuno-gold EM of mitochondrial proteins on cryo-sectioned cells.
From: Spatial orchestration of mitochondrial translation and OXPHOS complex assembly
Shown are representative EM images (right) and the quantifications taken from a larger number of images (left). The distributions of Cor1 and Cox2 were determined with antiserum against these proteins; all other sections were decorated with antiserum against GFP. Cells were grown at 30 °C in galactose containing medium. The arrowheads point to gold particles at the IBM (black) or at the CM (grey). For each analyzed protein localization, at least three individual slices were decorated and analyzed; n indicates the number of analyzed gold particles. Scale bars: 100 nm
