Supplementary Figure 3: DNA damage response and IRIF factor dependencies of FAM35A and C20orf196.
From: Shieldin complex promotes DNA end-joining and counters homologous recombination in BRCA1-null cells
a, Minimal variation of doxycycline induced GFP-FAM35A (U2OS) and GFP-C20orf196 (RPE1) in cells treated with the indicated siRNAs. Immunoblots shown are representative of two independent experiments with unprocessed scans of immunoblots in Supplementary Fig 8. b, Representative images of GFP-FAM35A (left panel) and GFP-C20orf196 (right panel) IRIF in γH2AX positive cells quantified in Fig 2e. Scale bar 10µm. c, Depletion of PTIP does not affect GFP-C20orf196 or GFP-FAM35A IRIF. Bars represent mean ± SEM, one-way Anova; ns=not significant (p≥0.05); n=3 independent experiments, with individual data points plotted over bars; statistical source data can be found in Supplementary Table 5. Scale bar 10µm. d, Camptothecin induced GFP-FAM35A foci. Scale bar 10µm. e, Representative images of GFP-FAM35A derivatives with/without pre-extraction ±IR; d-e representative of 2 independent experiments. Scale bar 10µm. f, Representative images of GFP-FAM35A N-terminus IRIF dependencies quantified in Fig 2f. Scale bar 10µm.
