Supplementary Figure 3: BAR proteins colocalizing with Endophilin in resting cells.

a, Confocal microscopy sections showing an example of the named BAR proteins tagged with EGFP at their C-termini colocalizing (RICH1-EGFP, left) or not (RICH2-EGFP, right) with endogenous Endophilin in BSC1 cells. b, Criteria used to score BAR candidates negative or positive. Intensity profiles were acquired along the indicated lines. c-f, Confocal microscopy sections showing the colocalization of the named controls (soluble EGFP and CAAX-EGFP), BAR, N-BAR or BAR-PH proteins tagged with EGFP at their C-termini and Endophilin. Images were cropped from larger pictures and angled as in (a) so that all images are oriented similarly. Arrowheads point to Endophilin spots colocalizing with BAR proteins at the cell surface. Images are representative of 10 captures from three independent biological experiments. All experiments were repeated independently at least three times with similar results. Scale bars, 5 μm.