Supplementary Figure 4: BAR proteins colocalizing with Endophilin in resting cells (continued).

a-c, Confocal microscopy sections showing the colocalization of the named PX-BAR, F-BAR or I-BAR proteins tagged with EGFP at their C-termini and Endophilin in BSC1 cells. Arrowheads point to Endophilin spots colocalizing with BAR proteins at the cell surface. Images are representative of 10 captures from three independent biological experiments. d, Absence of colocalization between internalized Alexa488-Transferrin and endogenous Endophilin in BSC1 and RPE1 cells stimulated with 10μM dobutamine and 50μg/mL Alexa488-Transferrin for 30sec at 37 °C for 30sec. Images are representative of 10 captures from three independent biological experiments. e, Top, β1-AR and GAPDH levels in the indicated plasma membrane and total fractions from BSC1 cells depleted or not for Endophilin (Endo TKD), AP2, FBP17 and CIP4 (F+C DKD) or Amphiphysin and Bin1 (A+B DKD). Unprocessed original scans are provided in Supplementary Fig. 6. f, Colocalization of β1-adrenergic receptor (β1-AR) and LAMP1 in resting BSC1 cells or upon incubation with 10μM dobutamine for 30min. Images are representative of 6 captures from three independent biological experiments. All experiments were repeated independently at least three times with similar results. Scale bars, 20 (f) and 5μm (a,,b,c,d).