Supplementary Figure 3: dumpy mutants show no difference in wing shape compared to wild-type wings at 18 hrs APF.

(a) Wing shape analysis of wild-type (top), and dumpy mutant (below) wings at 18 hrs APF. Scale bars, 50 μm. (b) Quantification of wild-type and dumpy mutant wing area shows no significant difference at 18 hrs APF. (two-tailed Mann-Whitney U test, p = 0.5476; n.s., n(wt) = 5 pupae and n(dp^ov1) = 5 pupae). (c) α−TUB and Arm in 18 hrs APF dp^ov1 wing show normal wing development at 18 hrs APF. (d) Left: Histogram of angular distribution of MTs in dp^ov1 wing show alignment of MT along P/D axis. Right: Quantification of MT alignment along P/D axis (Ordinary one-way ANOVA, from left to right: **** p < 0.0001, **** p < 0.0001, * p = 0.0107, n = 361 cells, 3 pupae). (e) MyoII is polarized in dumpy mutant wing at 18 hrs APF. MyoII-GFP in 18 hrs APF dp^ov1 wing show normal polarization pattern at 18 hrs APF. (f) Mean intensities of junctional MyoII as a function of the angle (θ) of the junctions with respect to the P/D axis. Right: Quantification of MyoII polarity (two-tailed Mann-Whitney U test, **** p < 0.0001; n(P/D) = 185 cells and n(A/P) = 107 cells, 4 pupae). (g) MyoII-GFP in 18 hrs APF wild-type wing. (h) Initial recoil velocities for the A/P and P/D oriented junctions show increased tension along P/D oriented junctions. (two-tailed t-test, ** p = 0.001; n(P/D) = 10 cells and n(A/P) = 10 cells, 5 pupae). Scale bars, (d, f, h left) 50 μm, (d, f, h right) 10 μm. Images shown in (a, c, e, g) are representative of n = 3 independent experiments. Boxes in all box plots extend from the 25^th to 75^th percentiles, with a line at the median. Whiskers show min and max values.