Supplementary Figure 4: Analyses of intended and non-intended base mutations in SC embryos from 4B2N1-DP2 cells and embryos obtained by injection of BE3 into zygotes. | Nature Cell Biology

Supplementary Figure 4: Analyses of intended and non-intended base mutations in SC embryos from 4B2N1-DP2 cells and embryos obtained by injection of BE3 into zygotes.

From: CRISPR–Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development

Supplementary Figure 4

a, SgRNA distribution in a total of 128 E12.5 SC embryos obtained by ICAHCI of 4B2N1-DP2 cells, showing that a majority of the SC embryos (89.8%) carried one sgRNA. b, SgRNA distribution in 115 embryos with one sgRNA, covering a total of 24 different sgRNAs. The horizontal axis represents the sgRNA number. The vertical axis represents the number of SC embryos with a specific sgRNA. c, Summary of the mutations in tested SC embryos with one sgRNA obtained through ICAHCI of 4B2N1-DP2 cells. Other represents the SC embryos without sgRNA or with 2 or more sgRNAs. d, Top, the number of identified sgRNAs in cell clones and SC embryos obtained from 4B2N1-DP2 cells. Bottom, the number of the sgRNAs that induced homozygous base mutations in cell clones and SC embryos determined by Sanger sequencing analyses. e, Blunt Cloning and sequencing analysis of different organs in one SC embryo carrying a heterozygous base mutation (top) and one with a homozygous base mutation (bottom). More than 20 clones were tested for each organ. f, Summary of the generation of E12.5 SC embryos from 4B2N1 cells carrying Dnd1 sgRNA libraries.

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