Supplementary Figure 6: Integrin inhibition does not exacerbate chemotherapy-induced toxicities.

Mice treated as described in Fig. 6d were a) weighed weekly. Body weights of treated mice did not differ significantly over the course of treatment or after one week, as assessed by multiple unpaired t tests and correcting for multiple comparisons using the Holm-Sidak method. Cohort size: IgG+AC: 11 animals; AIIB2+AC: 10 animals; AIIB2+LM709+AC: 9 animals. b) Bone marrow vascular density was determined from pan-laminin staining femurs of athymic nude females from the study described in Fig. 6c (Cohort 1). Data were not statistically significant, as determined by Kruskall-Wallis test and Dunn’s post-test to correct for multiple comparisons. c) Spleens from these mice were also weighed to grossly account for changes in immune cell content. Splenic weights did not differ between treatment conditions (but all were substantially lower than mice treated only with isotype control antibody), as assessed by one-way ANOVA and Dunnett’s post-test. For (b) and (c), Cohort size: IgG+AC: 4 animals; AIIB2+AC: 4 animals; AIIB2+LM709+AC: 3 animals. d) The effect of AIIB2 and/or AC therapy on immune cell content within bone marrow of uninoculated, immune-competent FVB mice was quantified by flow cytometery to determine whether AIIB2 affected specific immune cell populations. n = 3 animals were analyzed per treatment. No significant differences in viable CD45⁺ leukocytes were observed. *P = 0.029 and **P = 0.0096 when comparing the number of CD11b⁺ cells in IgG+AC and AIIB2+AC treated mice to untreated mice. **P = 0.0011 and ***P = 0.0008 when comparing the number of B220⁺ cells in IgG+AC and AIIB2+AC treated mice to untreated mice; *P = 0.033 when comparing the number of CD3⁺ cells in IgG+AC treated mice to untreated mice (comparing CD3⁺ cells in AIIB2+AC vs. untreated mice yielded P = 0.073). In each case, comparing IgG+AC and AIIB2+AC treatments yielded P values >0.983. All comparisons were made using one-way ANOVA and Dunnett’s post-test. For a-d, centre line represents the mean, error bars the s.e.m. Statistical tests and P values are indicated. Source data are provided in Supplementary Table 1.