Supplementary Figure 7: Expression of Snail or claudin-11 correlates with an advanced HNSCC and a worse prognosis.

(a) Immunohistochemical expression score of claudin-11 and Snail in adjacent normal tissues vs. HNSCC specimens. Y-axis displays the expression calculated by a modified H-score of claudin-11 (left) or Snail (right). The center values indicate the mean values. The p-value of each panel is shown (two-sided Student’s t test). N, normal; T, tumor. (b) Correlation between Snail expression and CTC counts in HNSCC patients. Left: correlation between Snail expression and clustering CTC counts. Right: correlation between Snail expression and single CTC counts. Each dot represents a HNSCC specimen (n = 21 patients). The Pearson correlation coefficient (r) and p-value are shown. (c) The correlation between the relative expression of SNAI1 and CLDN11 in 43 HNSCC specimens. Each red dot represents a specimen. The Pearson correlation coefficient (r) and p-value are shown. (d) Quantification of the relative CLDN11 (left) and SNAI1 (right) mRNA levels in tumor vs. normal parts of HNSCC patients. The p-value of each panel is shown (two-sided Student’s t test). N, normal; T, tumor. (e) The Kaplan-Meier survival plots to show the prognostic effect of CLDN11 expression in colorectal cancer (GSE17536) and ovarian cancer (DUKE-OC). The data were obtained from the public database PrognoScan. The corresponding p-value is indicated in each panel (two-sided Log-rank test). Source data is provided in Supplementary Table 11.