Supplementary Figure 5: PI4,5P2 binding controls the nuclear localization of p53.
From: A nuclear phosphoinositide kinase complex regulates p53
(a to f) A549 cells were transfected with the indicated siRNAs for 48 h and then treated with 30 μM cisplatin for 24 h before processed for IF staining of the indicated molecules. The images were taken with a Leica SP8 confocal microscope and processed by ImageJ. White arrows indicate the colocalized signals. The experiments were repeated three times and the graph is shown as mean ± SD of n = 10 cells from one representative experiment. Scale bar, 5 μm. Two-sided paired Student t-tests were used for statistical analysis. Statistical source data can be found in Supplementary Table 1. (g) Flag-p53 constructs were co-transfected with HA-PIPKIα in HEK293 cells. Flag-p53 proteins were immunoprecipitated and the associated PIPKIα was analyzed by IB with an anti-HA antibody. Representative data of three independent experiments were shown. Unprocessed images of the blots are shown in Supplementary Figure 9.
