Supplementary Figure 5: Glg1 localizes to the cytoplasmic membrane surface.

(a) Total lysate and membrane fractions from M1a cells were probed by western blot for Glg1 expression. Experiment performed once. (b) Immunoblot of M1a cell membrane lysates using E-selectin-IgG as a probe. (c) Immunoblot using anti-Glg1 as a probe of total cell lysates, immunoprecipitation supernatants, and E-selectin-immunoprecipitated proteins from each M1a cell line. Data representative of 2 independent experiments (b, c). Unprocessed original scans of the blots are shown Supplementary Fig. 9.