Supplementary Fig. 2: IRAK4-L and IRAK4-S activation of NF-κB and MAPK pathways. | Nature Cell Biology

Supplementary Fig. 2: IRAK4-L and IRAK4-S activation of NF-κB and MAPK pathways.

From: U2AF1 mutations induce oncogenic IRAK4 isoforms and activate innate immune pathways in myeloid malignancies

Supplementary Fig. 2

(a) Pathway analysis of enriched genes in AML patients preferentially expressing IRAK4-L (left) or IRAK4-S (right). Shown are the significant signalling networks and relevant genes associated with IRAK4-L or IRAK4-S expression in AML. Data is adapted from Fig. 2a. (b, c) HEK293 cells transfected with empty vector (pcDNA3.1), pcDNA3.1-IRAK4-L, or pcDNA3.1-IRAK4-S and then immunoblotted for NF-κB (p-IRAK1, p-p65) and MAPK (p-JNK, p-ERK, p-p38) signalling. (Three independent experiments). (d) HL60 cells, which express primarily IRAK4-S, were transduced with empty vector (pMSCV-pGK-GFP) or IRAK4-L and then immunoblotted for the indicated proteins. (Two independent experiments). (e) TF1 and HL60 cells were immunoblotted for IRAK4 with C-terminal antibody that detects IRAK4-L and IRAK4-S after immunoprecipitation of MyD88.

Back to article page