Supplementary Fig. 3: Rag GTPase-dependent mTORC1 translocation and GPR137B–mTOR interaction. | Nature Cell Biology

Supplementary Fig. 3: Rag GTPase-dependent mTORC1 translocation and GPR137B–mTOR interaction.

From: The lysosomal GPCR-like protein GPR137B regulates Rag and mTORC1 localization and activity

Supplementary Fig. 3

a, Representative images show that mTOR translocation is abolished in Rraga/b−/− MEFs. Scale bars, 20 μm. b, Repeat of mTOR, RagA interaction with GPR137B in MEFs and the requirement of Rag GTPases for the interaction of mTOR and GPR137B. mTOR and RagA co-immunoprecipitation with GPR137B-3×FLAG was examined in control and Rraga/b−/− MEFs stably expressing GPR137B-3×FLAG. Experiments in a and b were performed twice.

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