Supplementary Figure 5: Enhanced expression of IP3R1 reverts the defects in calcium signalling observed in IRE1α null cells. | Nature Cell Biology

Supplementary Figure 5: Enhanced expression of IP3R1 reverts the defects in calcium signalling observed in IRE1α null cells.

From: Non-canonical function of IRE1α determines mitochondria-associated endoplasmic reticulum composition to control calcium transfer and bioenergetics

Supplementary Figure 5

(A) IRE1α KO cells stably expressing CRa-IP3R1 or CRa-Control were imaged for calcium in the cytosol and mitochondria simultaneously with Fura2-AM and Rhodamine2N-AM (Rhod2). Integration of the maximum peak of individual cells from Fura2-AM and Rhod2-AM signals to calculate the non-linear regression k value and SEM (n = 5 independent experiments; total cells analysed: CRa-IP3R1: n = 87 cells; CRa-Control: n = 65 cells). (B) CRa-IP3R3 or CRa-Control were stained for proximity ligation assay (PLA) with anti-IP3R3 and anti-VDAC1 antibodies and visualized by confocal microscopy. Scale bar = 10 µm. Right panel: mean dots per cell were quantified (n = 2 independent experiments). Source data have been provided in Supplementary Table 6.

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