Supplementary Figure 6: Mesendoderm TFs are required for efficient exit from pluripotency.
From: Whsc1 links pluripotency exit with mesendoderm specification
a, siRNA knockdown efficiency was evaluated by RT-qPCR in Rex1GFPd2 cells 72 hrs after induction of exit from pluripotency. Data represent mean±s.d. from n=3 independent experiments and p-values were calculated by two-tailed unpaired t-test. b, Kinetics of GFP expression determined by FACS in Rex1GFPd2 ESCs transfected with siRNAs against T, Gata6, Gata4, Foxa2 at different times after differentiation induction. Data represent mean±s.d. from n=3 independent experiments.
