Supplementary Figure 2: Whsc1 depletion results in reduced mesendoderm differentiation in EpiSCs and in teratomas in vivo. | Nature Cell Biology

Supplementary Figure 2: Whsc1 depletion results in reduced mesendoderm differentiation in EpiSCs and in teratomas in vivo.

From: Whsc1 links pluripotency exit with mesendoderm specification

Supplementary Figure 2: Whsc1 depletion results in reduced mesendoderm differentiation in EpiSCs and in teratomas in vivo.

a, Expression of Whsc1, mesendoderm and ectoderm markers and Pou5f1 was quantified by RT-qPCR in control (shScr) and Whsc1 depleted (shWhsc1.2 and shWhsc1.4) EBs generated from EpiSCs (XGFP EpiSCs). Data represent mean±s.d. from n=3 independent experiments and p-values were calculated by two-tailed unpaired t-test. b, HE staining of teratomas from control (shScr) and Whsc1 depleted (Whsc1.2 and Whsc1.4) cells. Endodermal (gut), mesodermal (cartilage) and ectodermal (neuroepithelia) were indicated on the figures. Scale Bar: 200 μm. Ten teratomas from each group were examined, and representative images are shown. c, Expression of Whsc1, mesendoderm, ectoderm and pluripotency makers was quantified by RT-qPCR in control (shScr) and Whsc1 depleted teratomas (shWhsc1.2 and shWhsc1.4). Data represent mean±s.d. from n=3 independent experiments and p-values were calculated by two-tailed unpaired t-test.

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