Supplementary Figure 2: Correlation of m5C level with the oncogenic gene expression in bladder cancer samples.
From: 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs
a, Scatter plot showing the correlation of m5C methylation with gene expression levels for MLLT11 transcription factor 7 cofactor (MLLT11) and proteasome 26S subunit, non-ATPase 3 (PSMD3) in 65 tissue samples. The centre line and the shaded regions means the regression line and 95% confidence interval, respectively. Spearman's correlation coefficient and P value was calculated using the function “cor.test” in the statistical language R. b, c, Boxplot showing the distribution of m5C methylation level (b) and the mRNA expression (c) of MLLT11 and PSMD3 in tumours compared to matched normal samples. The P values were determined by a two-sided paired Wilcoxon signed rank test. d, Kaplan-Meier analysis indicating the correlation of the mRNA expression of MLLT11 and PSMD3 with poor DFS rates in UCB patients from TCGA analysis. The P values were determined by a two-sided log-rank test. e, Scatter plot showing the correlation of m5C methylation levels for HDGF, F11R, RAB11FIP4, MLLT11 and PSMD3 with NSUN2 gene expression level in 65 tissue samples. The centre line and the shaded regions means the regression line and 95% confidence interval, respectively. Spearman’s correlation coefficient and P value was calculated using the function “cor.test” in the statistical language R. In boxplots of b and c, central bars represent medians, box edges represent the first and third interquartile ranges. 22 pairs of cancerous and non-cancerous bladder tissues were used for b and c.
