Supplementary Figure 1: Further Characterization of Tau Condensates.
From: Microtubules gate tau condensation to spatially regulate microtubule functions
a, SDS-PAGE gel showing proteins used in this study. Protein constructs are listed to the right. N = 1 blot. b, Kymograph and images of the same MT as GFP-2N4R tau is added and subsequently washed out from the chamber. Scale bars: 2 μm (vertical), 2 min (horizontal). (left) and 10 min. (right). N = 2 chambers. c, Pixel intensity distribution of tau along MTs at various concentrations and MT types. WT (N) = 43, 57, 40, and 50. GMP-CPP (N) = 44, 96, and 82. Subtilisin (N) = 67, 52, 43, and 47 MTs, respectively. d, Distribution frequency of condensate lengths. N = 4, 52, 133, 233, 276, and 330 MTs for increasing tau concentrations respectively. e, Top: Intensity distribution of single TMR-SNAP-2N4R tau molecules. Fit to a Gaussian distribution is shown in red. N = 428 molecules from two independent trials. Bottom: Distribution of single- and two-step photobleaching observed for TMR-SNAP-2N4R tau molecules. Examples of single- and two-step photobleaching traces from the analysis. No molecules were observed to bleach in more than two steps. N = 49 molecules from 4 independent trials.
