Supplementary Figure 2: Developmental Regulation of Tau Localization in Cultured Neurons.
From: Microtubules gate tau condensation to spatially regulate microtubule functions
a, Images of mouse hippocampal neurons at different days cultured in vitro. Neurons were immunostained with two different pan-tau antibodies, GTX49353 (Genetex) and T46 (Thermo Fisher). Scale bar: 25 μm. N = 4 preparations of neurons. b, Quantification of tau puncta frequency in neuron processes. Data represented the mean ± SD. N = 18, 26, and 23 images (from ≥ 3 different neuron preps) for DIV3, DIV4, and DIV7, respectively.
