Supplementary Figure 3: The C-terminal Pseudo-Repeat Region of Tau Licenses the Rest of the Molecule Into Tau Condensates.
From: Microtubules gate tau condensation to spatially regulate microtubule functions
a, Schematic of tau isoforms and constructs. Orange boxes: alternatively spliced N-term. inserts. Blue: proline-rich domain. Green: MT binding repeats. Yellow: pseudo-repeat domain. b, Top: Images of tau isoforms incorporating into full-length tau (2N4R) condensates. Middle: intensity plots of both channels. Bottom: tau isoform intensities on the lattice and within 2N4R condensates. Scale bar: 2 μm. N = 176 and 211 continuous MT segments (3 chambers each) for 2N4R. N = 127 and 143 segments (3 chambers) for 2N3R. 309 and 158 segments (3 chambers) for 0N3R. c, Top: images of constructs incorporating in 2N4R-tau condensates. Middle: intensity plot of both channels. Bottom: isoform intensities on the lattice and in condensates. Scale bar: 2 μm. N = 152 and 119 segments (3 chambers) for MTBD, 189 and 208 segments (4 chambers) for Proj., 182 and 149 segments (4 chambers) for C-Term, 337 and 296 segments (6 chambers) for bonsai. d, Top: images of Mini-Tau and pseudo-repeat truncations incorporating into 2N4R-tau condensates. Middle: intensity plot of both channels. Bottom: tau isoform intensities on the lattice and in condensates. Scale bar: 2 μm. N = 144 and 239 segments (3 chambers) for Mini-Tau, 145 and 122 segments (3 chambers) for Mini-TauΔ9, 120 and 75 segments (4 chambers) for Mini-TauΔ28. Data represented the mean ± SD. For b, c, and d Student’s T-test (two sided). e, Sequence alignments showing identity conservation (top) and hydrophobicity (bottom) of the tau pseudo-repeat region. The pseudo-repeat is highlighted by green box above.
