Extended Data Fig. 5: Mutant histone expression impacts HSC gene expression. | Nature Cell Biology

Extended Data Fig. 5: Mutant histone expression impacts HSC gene expression.

From: Inducible histone K-to-M mutations are dynamic tools to probe the physiological role of site-specific histone methylation in vitro and in vivo

Extended Data Fig. 5

(a) GSEA analysis based on RNA-seq data for hematopoietic stem and progenitor cells sorted from H3K9M and H3K36M mice induced for 4 weeks (H3, n = 2; H3K9M, n = 2; H3K36M, n = 2). Enrichment is shown for transcriptional signatures related to Lymphoid/Myeloid progenitors (CMP, LMPP, and proB)33 and B cells50 (K9M) as well as erythroid cells33 and B cells50 (K36M). Statistics were generated in accordance with the published GSEA algorithm51. (b) Principal component analysis based on the union of differentially expressed genes between rtTA control and H3K9M or H3K36M samples. Each circle represents a biological replicate (rtTA, n = 3; H3K9M, n = 3; H3K36M, n = 3) while squares represent the mean. (c) Expression (RPKM) values for select genes relative to average expression in rtTA replicates (shown in log2 scale) for biological triplicates from LKS cells sorted at day 7 of induction.

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