Extended Data Fig. 1: Expression of H3K9M and H3K36M suppresses methylation and blocks differentiation of pluripotent stem cells. | Nature Cell Biology

Extended Data Fig. 1: Expression of H3K9M and H3K36M suppresses methylation and blocks differentiation of pluripotent stem cells.

From: Inducible histone K-to-M mutations are dynamic tools to probe the physiological role of site-specific histone methylation in vitro and in vivo

Extended Data Fig. 1

(a) Western blot analysis for tri-methyl H3K9 and H3K36 with and without mutant histone expression in mouse ES cells. (b) Pie charts showing the distribution of ATAC-seq peaks in EBs expressing H3, H3K9M, and H3K36M. (c) Images of teratomas derived from ES cells expressing H3, H3K9M, or H3K36M. Scale bar = 200 μm; endo = endoderm, meso = mesoderm, ecto = ectoderm. (d) qRT-PCR analysis for knockdown of H3K9 and H3K36 KMTs. One sample is shown. (e) Flow cytometry for Rex1-GFP at days 2 and 3 of embryoid body formation. (f) Western blot analysis showing H3K36me3 levels following knockdown of H3K36 KMTs. (g) Western blot analysis showing H3K9me3 levels following knockdown of H3K9 KMTs. See source data for full membrane Western blot images. Data in a,c,e,f,g are representative of 3 independent experiments.

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