Extended Data Fig. 4: Flow cytometry analysis reveals the presence of endothelial and haematopoietic cells expressing GFP in the E16-grafted chicken BM.
Distribution of CD45, MEP21, CD41, c-KIT, and CD3 populations in grafted (a, b) or wild-type (c, d) BM mononucleated cells. a, n = 12 independently grafted animals were used for CD45, CD41, c-KIT and CD3 expression and n = 8 for MEP21. b, Representative flow cytometry analysis of an E16/E17 grafted BM showing that GFP+ cells derived from the PSM graft contributed to all lineages. Numbers in green indicate the percentage of GFP+ cells in each population (that is in CD45+, MEP21+, CD41+, c-KIT+ and CD3+ populations). c, n = 3 independent WT animals were used for CD45, CD41, c-KIT expression analysis, n = 2 animals were used for MEP21 and CD3 expression analysis. d, Representative flow cytometry analysis of an E16/E17 WT BM. Data in b representative of 4 independent experiments, 3 in d. Error bars are mean ± SEM.
