Extended Data Fig. 6: Flow cytometry analysis reveals the presence of endothelial and haematopoietic cells expressing GFP in adult BM grafted chickens.
Distribution of CD45, MEP21, CD41, c-KIT, CD3 and KUL1 populations in grafted (a, b) and wild-type (c, d) adult BM mononucleated cells. a, Grafted chickens. CD45 (n = 7), MEP21 (n = 4), CD41 (n = 7), c-KIT (n = 7), CD3 (n = 7) and KUL1 (n = 6) independent chickens were used for the analysis. b, Representative multilineage flow cytometry analysis of a grafted adult BM showing that GFP+ cells derived from the PSM graft contributed to all lineages. Numbers in green indicate the percentages of GFP+ cells in each population (that is in CD45+, MEP21+, CD41+, c-KIT+, CD3+ and KUL1+ populations). c, Wild-type animals. CD45 (n = 5), CD41 (n = 4), c-KIT (n = 5), CD3 (n = 4) and KUL1 (n = 4); independent animals were used for the analysis. n = 2 animals were used for MEP21 expression analysis. d, Representative multilineage flow cytometry analysis of a wild-type adult BM showing the absence of GFP staining. Error bars represent mean ± SEM. Data in b and d representative of 4 independent experiments.
