Extended Data Fig. 10: Long-term repopulation analysis in the peripheral blood of recipients transplanted either with YFP− or YFP+ LSK cells. | Nature Cell Biology

Extended Data Fig. 10: Long-term repopulation analysis in the peripheral blood of recipients transplanted either with YFP− or YFP+ LSK cells.

From: In vivo generation of haematopoietic stem/progenitor cells from bone marrow-derived haemogenic endothelium

Extended Data Fig. 10: Long-term repopulation analysis in the peripheral blood of recipients transplanted either with YFP− or YFP+ LSK cells.

a, b, Flow cytometry analysis of circulating blood from 6 (a) and 4 (b) C57Bl/6-CD45.1 recipient mice at 11 weeks injected with 2,000 YFP− (a) or YFP+ (b) LSK cells (C57Bl/6-CD45.2), respectively. Mice recipients transplanted with YFP− LSK cells displayed a more robust reconstitution than the ones transplanted with YFP+ LSK cells. Of note, two mice in the LSK YFP+ series died before two weeks post-injection. c, Back-gating within the GFP+ population of the mononucleated bone marrow cells from mouse #d at 14 weeks of reconstitution. Dot plot representation of flow cytometry multilineage analysis showing Gr1 (granulocytes), CD11b (macrophages/monocytes), B220 and CD19 (B cells), CD4 (T cells), CD8 (T cells), Ter119 and CD71 (red cells), CD41 and CD61 (megakaryocytes) staining. The YFP population was more prominent in B220, CD19, CD71, Gr1 and CD8 fractions. Data in a-c representative of 2 independent experiments.

Back to article page