Extended Data Fig. 3: TBK1 mediates mTORC1 activation by EGF and has cell type-specific functions in mTORC1 activation by TLR ligands.
From: TBKBP1 and TBK1 form a growth factor signalling axis mediating immunosuppression and tumourigenesis
a, Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of A549 cells stably infected with a control shRNA (Ctrl) or two different Tbk1-specific shRNAs (D5 and D9), stimulated with Pam3Csk4. b-h, Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of primary lung cells from WT and Tbk1cKO mice (b),Tbk1WT-KrasLA2 (WT-Kras) and Tbk1cKO-KrasLA2 (cKO-Kras) mice (c,d), bone marrow-derived macrophages (BMDM) from WT or Tbk1 myeloid cell-conditional KO (MKO) mice (e,f), or WT and Tbk1 KO primary MEFs (g,h), stimulated with the indicated TLR ligands. Data are representative of three independent experiments. Unprocessed blots are shown in Unprocessed Blots Fig. 3.
