Extended Data Fig. 5: TANK and NAP1 are dispensable for EGF-induced mTORC1 activation.
From: TBKBP1 and TBK1 form a growth factor signalling axis mediating immunosuppression and tumourigenesis
a-d, Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of A549 cells stably infected with a control shRNA (shCtrl) or shRNAs specific for Tank (a), Nap1 (b), or Tbkbp1 (c,d), stimulated with the indicated inducers. e,f, Genotyping PCR (e) and immunoblot (f) analyses of Tbkbp1+/+Ccsp-Cre (WT), Tbkbp1+/flCcsp-Cre (Het), and Tbkbp1fl/flCcsp-Cre (cKO) mice, showing the flox and WT Tbk1 alleles and Ccsp-Cre (e) and the TBKBP1 protein expression (f). Data are representative of three independent experiments. Unprocessed blots are shown in Unprocessed Blots Fig. 5.
