Extended Data Fig. 7: TBK1 deletion in lung epithelial cells promotes T cell activation.
From: TBKBP1 and TBK1 form a growth factor signalling axis mediating immunosuppression and tumourigenesis
a, Immunohistochemical staining of T cells using the indicated antibodies in lung sections of Tbk1WT-KrasLA2 (WT-Kras) and Tbk1cKO-KrasLA2 (cKO-Kras) mice. Scale bar, 100 mm. Data are presented as a representative image (upper) or summary graphs (lower, n = 7 mice per group). b,c, Flow cytometric analysis of the frequency of CD4 and CD8 T cells in lung cells (b) or IFNg+ effector T cells within lung CD4 or CD8 T cell populations (c). n = 5 mice per group. d-g, Experimental design (d), efficiency of T cell depletion in the lung and spleen (e), a representative image of lung tumours (f, scale bar, 100 mm), and summary graphs of lung weight, tumour numbers, and tumour size (g) of Tbk1WT-KrasLA2 and Tbk1cKO-KrasLA2 mice treated with anti-CD3 or an IgG isotype control (150 mg/mouse). n = 11 mice per group. Data are representative of three independent experiments, and bar graphs are presented as mean±s.d. values. Two-sided unpaired Student’s t-test (a,b,c,g). Source data for graphs are provided in Statistical Source Data Fig. 7.
