Extended Data Fig. 1: TBKBP1 is not required for TBK1 activation or type I IFN induction by TLR ligands and viruses.
From: TBKBP1 and TBK1 form a growth factor signalling axis mediating immunosuppression and tumourigenesis
a, Immunoblot analysis of the indicated phosphorylated (P-) and total proteins in whole-cell lysates of A549 cells stably infected with a control shRNA or a Tbkbp1-specific shRNA, stimulated with the indicated inducers. b-e, qRT-PCR analysis of Ifna and Ifnb mRNA expression in A549 cells stably infected with a control shRNA (shCtrl) or shRNAs for Tbk1 (b,c) or Tbkbp1 (d,e), stimulated with the indicated inducers. Data are representative of three independent experiments. n = 3 (b,d,e) or 5 (c) per group. Two-sided unpaired Student’s t-test (b-e). Source data for graphs are provided in Statistical Source Data Fig. 1 and unprocessed blots are shown in Unprocessed Blots Fig. 1.
