Extended Data Fig. 2: TBK1, but not IKKε, mediates growth factor-stimulated mTORC1 activation.
From: TBKBP1 and TBK1 form a growth factor signalling axis mediating immunosuppression and tumourigenesis
a, Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of freshly isolated lung cells from Tbk1WT-KrasLA2 and Tbk1cKO-KrasLA2 mice. b-e, Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of control or Tbk1-knockdown H157 (b), H460 (c), or A549 (d,e) cells, stimulated with the indicated inducers. f, g, Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of WT or Tbk1-deficient primary MEF cells stimulated by EGF (f) or essential amino acids (EAAs, g). h, Immunoblot analysis of the indicated proteins in the cytoplasmic extracts (Cyt Ext) or nuclear extracts (Nucl Ext) of control or Tbk1-knockdown A549 cells, stimulated with EGF. i, Immunoblot analysis of the indicated phosphorylated (p-) and total proteins in whole-cell lysates of EGF-stimulated A549 cells stably infected with a control shRNA (shCtrl) or two different IKKe-specific shRNAs. Data are representative of three independent experiments. Unprocessed blots are shown in Unprocessed Blots Fig. 2.
