Extended Data Fig. 5: Single cell RNA sequencing identified EMP-derived osteoclast and their precursor populations. | Nature Cell Biology

Extended Data Fig. 5: Single cell RNA sequencing identified EMP-derived osteoclast and their precursor populations.

From: Erythromyeloid progenitors give rise to a population of osteoclasts that contribute to bone homeostasis and repair

Extended Data Fig. 5

a, t-SNE plot of tdTomato+Tnfrsf11a+Ptprc+ cells (n=3,368 cells) from Csf1r-Mer-iCre-Mer; Csf1reGFP; R26tdTomato and Cx3cr1creER; R26tdTomato mice identifying 13 clusters. The right gray rectangle representing the normalized expression of indicated marker genes visualized onto t-SNE plots. Cells in cluster 9 expressed osteoclast-specific marker genes. EMP, erythromyeloid progenitor. b, Heatmap representing the top 10 significantly differentially expressed genes in each cluster. c, Violin plots showing mRNA expression levels of selected marker genes. Green color showing the expression levels of the cells from Cx3cr1creER; R26tdTomato mice. Blue color showing the expression levels of the cells from Csf1r-Mer-iCre-Mer; Csf1reGFP; R26tdTomato mice. The number shows cluster identity, and the number of cells in each cluster is provided in Statistical source data Extended Data Fig 5. d, Selected pathways significantly enriched in the cells belonging cluster 6 (n=274 cells). Hypergeometric probability mass function was used. Statistics source data are provided in source data Extended Data Fig 5.

Back to article page